Imaging:Article Title: Direct visualization of DNA affinity landscapes using a high-throughput sequencing instrument
Article Snippet: .. Double stranded DNA generation 4.1 To avoid the delivery of scan mix before protein imaging, the ImageCyclePump.xml config file (usually within C:\Illumina\SCS2.6\DataCollection\bin\Config) was modified as follows: “ On=“true” AutoDispense=“false”>” was changed to “”. .. 4.2 1uM of the primer used for dsDNA generation (5’ /5Alex647N/-ACACTCTTTCCCTACACGACGCTCTTCCGATCT 3’) was manually hybridized using receipt “GA2_Manual_ReHyb_v7.xml.” 4.3 The reagent on location 2 of GA was replaced with 1X NEB buffer 2/0.01% Tween buffer: 10x NEB buffer 2 100ul 10% Tween 1ul H2O 899ul Total 1000ul Open in a separate window 4.4 100ul of 1X NEB buffer 2/0.01% Tween buffer were manually delivered at a rate of 100ul/min from location 2 of GA. 4.5 The reagent on location 2 of GA was replaced with with Resynthesis Mix: 10X NEB buffer 2 100ul 25mM dNTP mix 10ul Klenow enzymev(NEB, 5U/ul) 20ul 10% TWEEN 1ul H2O 869ul Total 1000ul Open in a separate window 4.6 100ul of Resynthesis Mix were manually delivered at a rate of 100ul/min from location 2 of GA. 4.7 The Peltier temperature controller of GA was set to 37C via the manual control tab, and kept at 37C for 30 minutes.
Article Title: Direct measurement of DNA affinity landscapes on a high-throughput sequencing instrument.
Article Snippet: .. To avoid the delivery of scan mix before protein imaging, the ImageCyclePump.xml config file (usually within C:\Illumina\SCS2.6\ DataCollection\bin\Config) was modified as follows: “ On=”true” AutoDispense = ”false”>” was changed to “”. .. 1 μM of the primer used for dsDNA generation (5′ /5Alex647N/-ACACTCTTTCCCTACACGACGCTCTTCCGA TCT 3′) was manually hybridized using recipe “GA2_Manual_ReHyb_v7.xml.” The reagent on location 2 of GA was replaced with 1× New England Biolabs (NEB) buffer 2/0.01% Tween buffer (10× NEB buffer 2 100 μl; 10% Tween 1 μl; H2O 899 μl; total vol.
Article Title: Direct visualization of DNA affinity landscapes using a high-throughput sequencing instrument
Article Snippet: .. 4.1 To avoid the delivery of scan mix before protein imaging, the ImageCyclePump.xml config file (usually within C:\Illumina\SCS2.6\DataCollection\bin\Config) was modified as follows: “ On=“true” AutoDispense=“false”>” was changed to “”. .. 4.2 1uM of the primer used for dsDNA generation (5’ /5Alex647N/-ACACTCTTTCCCTACACGACGCTCTTCCGATCT 3’) was manually hybridized using receipt “GA2_Manual_ReHyb_v7.xml.” 4.3 The reagent on location 2 of GA was replaced with 1X NEB buffer 2/0.01% Tween buffer: 10x NEB buffer 2 100ul 10% Tween 1ul H2O 899ul Total 1000ul Open in a separate window 4.4 100ul of 1X NEB buffer 2/0.01% Tween buffer were manually delivered at a rate of 100ul/min from location 2 of GA. 4.5 The reagent on location 2 of GA was replaced with with Resynthesis Mix: 10X NEB buffer 2 100ul 25mM dNTP mix 10ul Klenow enzymev(NEB, 5U/ul) 20ul 10% TWEEN 1ul H2O 869ul Total 1000ul Open in a separate window 4.6 100ul of Resynthesis Mix were manually delivered at a rate of 100ul/min from location 2 of GA. 4.7 The Peltier temperature controller of GA was set to 37C via the manual control tab, and kept at 37C for 30 minutes.
Modification:Article Title: Direct visualization of DNA affinity landscapes using a high-throughput sequencing instrument
Article Snippet: .. Double stranded DNA generation 4.1 To avoid the delivery of scan mix before protein imaging, the ImageCyclePump.xml config file (usually within C:\Illumina\SCS2.6\DataCollection\bin\Config) was modified as follows: “ On=“true” AutoDispense=“false”>” was changed to “”. .. 4.2 1uM of the primer used for dsDNA generation (5’ /5Alex647N/-ACACTCTTTCCCTACACGACGCTCTTCCGATCT 3’) was manually hybridized using receipt “GA2_Manual_ReHyb_v7.xml.” 4.3 The reagent on location 2 of GA was replaced with 1X NEB buffer 2/0.01% Tween buffer: 10x NEB buffer 2 100ul 10% Tween 1ul H2O 899ul Total 1000ul Open in a separate window 4.4 100ul of 1X NEB buffer 2/0.01% Tween buffer were manually delivered at a rate of 100ul/min from location 2 of GA. 4.5 The reagent on location 2 of GA was replaced with with Resynthesis Mix: 10X NEB buffer 2 100ul 25mM dNTP mix 10ul Klenow enzymev(NEB, 5U/ul) 20ul 10% TWEEN 1ul H2O 869ul Total 1000ul Open in a separate window 4.6 100ul of Resynthesis Mix were manually delivered at a rate of 100ul/min from location 2 of GA. 4.7 The Peltier temperature controller of GA was set to 37C via the manual control tab, and kept at 37C for 30 minutes.
Article Title: Direct measurement of DNA affinity landscapes on a high-throughput sequencing instrument.
Article Snippet: .. To avoid the delivery of scan mix before protein imaging, the ImageCyclePump.xml config file (usually within C:\Illumina\SCS2.6\ DataCollection\bin\Config) was modified as follows: “ On=”true” AutoDispense = ”false”>” was changed to “”. .. 1 μM of the primer used for dsDNA generation (5′ /5Alex647N/-ACACTCTTTCCCTACACGACGCTCTTCCGA TCT 3′) was manually hybridized using recipe “GA2_Manual_ReHyb_v7.xml.” The reagent on location 2 of GA was replaced with 1× New England Biolabs (NEB) buffer 2/0.01% Tween buffer (10× NEB buffer 2 100 μl; 10% Tween 1 μl; H2O 899 μl; total vol.
Article Title: Direct visualization of DNA affinity landscapes using a high-throughput sequencing instrument
Article Snippet: .. 4.1 To avoid the delivery of scan mix before protein imaging, the ImageCyclePump.xml config file (usually within C:\Illumina\SCS2.6\DataCollection\bin\Config) was modified as follows: “ On=“true” AutoDispense=“false”>” was changed to “”. .. 4.2 1uM of the primer used for dsDNA generation (5’ /5Alex647N/-ACACTCTTTCCCTACACGACGCTCTTCCGATCT 3’) was manually hybridized using receipt “GA2_Manual_ReHyb_v7.xml.” 4.3 The reagent on location 2 of GA was replaced with 1X NEB buffer 2/0.01% Tween buffer: 10x NEB buffer 2 100ul 10% Tween 1ul H2O 899ul Total 1000ul Open in a separate window 4.4 100ul of 1X NEB buffer 2/0.01% Tween buffer were manually delivered at a rate of 100ul/min from location 2 of GA. 4.5 The reagent on location 2 of GA was replaced with with Resynthesis Mix: 10X NEB buffer 2 100ul 25mM dNTP mix 10ul Klenow enzymev(NEB, 5U/ul) 20ul 10% TWEEN 1ul H2O 869ul Total 1000ul Open in a separate window 4.6 100ul of Resynthesis Mix were manually delivered at a rate of 100ul/min from location 2 of GA. 4.7 The Peltier temperature controller of GA was set to 37C via the manual control tab, and kept at 37C for 30 minutes.
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